Application of synthetic peptides in development of a serologic method for laboratory diagnosis of schistosomiasis mansoni.

Development of a Serologic Method for Laboratory Diagnosis of the Schistosomiasis Mansoni Based on Synthetic Peptides

Based on algorithms of hidrophilicity and flexibility, obtained by the use of the ProtScale software, disposable at the site http:// au.expasy.org/cgi-bin/protscale.pl, seven potentially antigenic peptides were selected from different Schistosoma mansoni proteins: cathepsin B (Sm31), heat shock protein (SmHSP-70), cathodic circulating antigen (CCA) and the polypeptidic sequence of the open read...

Schistosomiasis serology is valuable and reliable.

TO THE EDITOR—We read the photo quiz authored by Bagdure and Khasawneh with interest [1]. The authors described a case of schistosomiasis colitis caused by Schistosoma mansoni, which was diagnosed after review of hematoxylin-eosin– stained colonic biopsy sections. This case emphasizes the challenges associated with obtaining a laboratory diagnosis of schistosomiasis based on examination of ova ...

Use of synthetic peptides derived from adult worm proteins of Schistosoma mansoni, in the diagnosis of schistosomiasis.

Mast Cells Kinetics during Experimental Schistosomiasis mansoni in Mice

Increased number of mast cells at the site of infection is widely regarded as important host defense against parasites. The kinetics of mucosal mast cells and connective tissue mast cells responses were studied in the intestines of 68 female CFLP mice infected with 100 Schistosoma mansoni cercariae. The number of mucosal mast cells and the connective tissue mast cells increased from week 3 and ...

[IgM-ELISA for diagnosis of schistosomiasis mansoni in low endemic areas].

An immunoenzymatic method for the detection of IgM antibodies (IgM-ELISA) against a fraction of Schistosoma mansoni adult worm antigen, soluble in trichloroacetic acid (TCA-soluble fraction), was evaluated for epidemiological purposes in low endemic areas for schistosomiasis. Blood samples on filter paper were collected from a population living in the municipality of Pedro de Toledo, São Paulo ...